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(Positions Available in Proteomics and Mass Spectrometry)
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| Education and Training |
- Gannan Teachers' college, Ganzhou, BSc
- Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, MSc (Analytical Chemistry)
- Institute of Chemistry, Chinese Academy of Sciences, Beijing, PhD (Analytical Biochemistry)
- University of Kentucky, Lexington, Postdoctoral Fellow (Cancer Proteomics)
- Children's National Medical Center, Washington, DC, Research Associate (Cancer Proteomics)
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| Research Interest - Protein Mass Spectrometry and Cancer Proteomics |
| My interests lie in: 1) development of novel methods, particularly mass spectrometric and proteomic methods, to address the difficulties in biomedical research; and 2) Identification of the protein markers for tumor metastasis and progression. I am particularly interested in the plasma membrane proteins and metal binding proteins. |
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| - Plasma Membrane Proteins |
| The plasma membrane of cancer cells is enriched with receptors that potentially are drug targets. However, these proteins are among the most difficult cellular molecules to characterize by the current technologies, e.g. chromatography or mass spectrometry. I have developed a so called “Tube-gel” technique that facilitates the solubilization and high throughput LC-MS/MS analysis of membrane proteins (Lu and Zhu, Mol. Cell. Proteomics, 2005). In addition, I have been developing novel strategies of affinity chromatography to selectively isolate and enrich plasma membrane proteins. With these techniques, I was able to selectively profile the surface membrane proteins of cultured prostate cells. The preliminary studies on the comparison of the plasma membrane proteins of prostate cancer cells (PC-3 and LnCAP) with benign cells (BPH-1) revealed an array of differentiated plasma membrane proteins, such as integrin complexes, tranferrin receptor, and insulin-like growth factor. I’d like to further define the approaches and apply the developed techniques to dissect the plasma membrane proteome associated with tumor progression of animal models. |
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| - Human Metallothionein |
| Metallothioneins (MTs) are a group of low molecular weight (6 ~ 7 kDa) cysteine rich, intracellular metal binding proteins. They are inducible by a variety of factors such as heavy metals and oxidative stresses. MT has a high affinity for binding to transition heavy metals like Zn, Cu and Cd, attributed by their high content of cysteine residues (20/21 Cys out of total 60 ~ 70 amino acid residues). The commonly accepted function of MTs is detoxification of heavy metals, particularly Cd. Plenty of studies also have shown MTs are up-regulated or over expressed in numerous human cancers with progression associated with poor prognosis. MTs are thus potential protein marker for tumor progression. Unfortunately, the MTs in humans comprise of at least 10 functional isoforms and there has been no method that can distinguish all the individual protein isoform of MTs due to the challenges with the highly conserved sequences of MTs. I am interested in establishing mass spectrometry-based proteomic approaches to identify and characterize the specific human MT isoforms to the progression of cancers. In collaboration with Dr. D. Sens, an established expert in the research of MT toxicology and biology, I have made significant progress in methodology development for the identification of MT isoforms in humans. These include a simple and rapid method for isolation and identification of human MT isoforms in cultured cells using MALDI-TOF/TOF mass spectrometry (Analytical Chemistry, 2007, in press). |
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| Recent Publications, Patent & Conferences |
| Peer-Reviewed Journal Articles (* indicates corresponding author) |
- Rongying Wang, Donald A. Sens, Amy Albrecht, Scott Garrett, Seema Somji, Mary Ann Sens, and Xiaoning Lu*, Simple Method for Identification of Metallothionein Isoforms in Cultured Human Prostate Cells by MALDI-TOF/TOF Mass Spectrometry, Analytical Chemistry, 2007, 79, 4433-4441. [PDF]
- Rongying Wang, Donald A. Sens, Scott Garrett, Seema Somji, Mary Ann Sens, and Xiaoning Lu*, The Resistance of Metallothionein to Proteolytic Digestion: A Liquid Chromatography/Tandem Mass Spectrometric Analysis, Electrophoresis, August 2007, in press.
- Enantioselective Oxidation of Trans-4-Hydroxy-2-Nonenal is ALDH Isozyme and Mg2+-Dependent, Jiri Brichac, Kwok Ki Ho, Ales Honzatko, Rongying Wang, Xiaoning Lu, Henry Weiner, Matthew J. Picklo, Sr.
Chemical Research in Toxicology, 2007, 20, 887-895. [PDF]
- EunKyung An, Xiaoning Lu, Jessica Fllipin, Joe Devaney, Brian Halligan, Eric Hoffman, Karl Casky and Yetrib Hathout, Secreted Protein Profiling in Human RPE Cell Cultures Derived from Postmortem Age Related Macular Degeneration Donors and Age Matched Healthy Donors. Journal of Proteome Research, 2006, 5(10): 2599-2610. [PDF], (Featured by the same journal)
- Xiaoning Lu and Haining Zhu, Tube-Gel digestion: A novel proteomic approach for high throughput analysis of membrane proteins, Molecular & Cellular Proteomics, 2005, 4 (12): 1948-1958. [PDF]
- Yi Chen, Xiaoning Lu, Zhiqiang Han, et. al. Chiral ion-exchange capillary electrochromatography of arylglycine amides with dextran sulfate as a pseudostationary phase. Electrophoresis, 2005, 26 (4-5): 833-840. [PDF]
- Xiaoning Lu, Yi Chen, Lei Guo and Yifang Yang, Chiral separation of underivatized amino acids by ligand-exchange capillary electrophoresis using a copper(II)-L-lysine complex as selector, Journal of Chromatography A, 2002, 945(1/2): 249-255. [PDF]
- Xiaoning Lu and Yi Chen, Chiral separation of amino acids derivatized with fluoresceine-5-isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of ?-cyclodextrin and sodium taurocholate, Journal of Chromatography A, 2002, 955(1): 133-140. [PDF]
- Rongying Wang, Xiaoning Lu, Ma Wanyun, Determination of fetoprotein by non-competitive capillary electrophoresis immunoassay, Journal of Chromatography B, 2002, 779(2): 157-162. [PDF]
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| Submitted and In-preparation manuscript |
- Rongying Wang, Donald A. Sens, Amy Albrecht, Scott Garrett, Seema Somji, Mary Ann Sens, and Xiaoning Lu*, Identification of Metallothionein Isoforms by Nano-Flow Liquid Chromatography and Tandem Mass Spectrometry: An comparison to MALDI-TOF/TOF Mass Spectrometry. (In preparation).
- Xiaoning Lu, Zhenyu Huang, Fujian Zhang, Kei Fukada, Haining Zhu and Natasha Kyprianou, Cell surface proteomics of prostate cancer by affinity chromatography and tandem mass spectrometry (in preparation).
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| Patent |
- Mingjia, Wu, Xiaoning Lu and Rongying Wang, "A novel amperometric detection cell for capillary electrophoresis" awarded on June 2002. Patent Number: 98215644.8 (China).
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Conferences
- 54th ASMS, May 28 - June 1, 2006, Seattle, WA |
- Xiaoning Lu, Yetrib Hathout, Tobey MacDonald and Brian Rood, Toward Biomarker Discovery for Childhood Brain Tumors: a Proteomic Study of Cerebrospinal Fluids (poster, MP621).
- Eunkyung An, Xiaoning Lu, Jessica Flippin, Joe Devaney, Brian Halligan, Karl Csaky, Yetrib Hathout, Comparative Secretomics in RPE Cells from Normal and Age Related Macular Degeneration Donors (poster, TP576).
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| - 53rd ASMS, June 5 - 9, 2005 San Antonio, TX |
- Xiaoning Lu, Kei Fukada; Alexis Vien, Haining Zhu, A novel proteomic approach for high throughput membrane protein analysis (Oral presentation).
- Xiaoning Lu, Haining Zhu, Biotinylation signatures for the unambiguous identification of the cell surface proteins of prostate cancer cells (Poster WP542).
- Kei Fukada, Alexis Vien, Xiaoning Lu, Fujian Zhang, Haining Zhu, Improvement of quantitative proteomics with 18oxygen incorporation: from sample preparation to data processing (Poster WP505).
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| - 52nd ASMS, May 23 - 27, 2004, Nashville, TN |
- Xiaoning Lu, Fujian Zhang, Li Liu, Kei Fukada, Haining Zhu, LC-MS/MS Analysis of Prostate Cancer Cell Surface Proteome (TPY457 poster).
- Kei Fukada; Xiaoning Lu; Theresa M. Guerin; William V. Everson; Eric J. Smart; Haining Zhu, Proteomic Analysis of Lipid Rafts from Mouse Monocytes (Poster MPT336).
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